aza 1h benzotriazole 1 yl 1 1 3 3 tetramethyluronium hexafluorophosphate hatu  (Chem Impex International)

Journal: Physiological Reports

Article Title: Induction of cardiac alternans in human iPS ‐derived cardiomyocytes through β‐adrenergic receptor stimulation

doi: 10.14814/phy2.70152

Figure Lengend Snippet: Experimental condition and observed responses of cardiac cell sheet tissues with C‐ALT exposed to each compound.

Article Snippet: Isoproterenol (I0260; Tokyo Chemical Industry, Tokyo, Japan), propranolol (163‐24501; Fujifilm Wako Pure Chemical Corporation), verapamil (222‐00781; Fujifilm Wako Pure Chemical Corporation), ryanodine (ab120083; Abcam, Cambridge, UK), thapsigargin (10522; Cayman Chemical, MI, USA), omecamtiv mecarbil (CS‐0460; Chemscene LCC, NJ, USA), ivabradine (098–06921; Fujifilm Wako Pure Chemical Corporation), and blebbistatin (ax494682; Axol Bioscience) were dissolved in DMSO (031‐24051; Fujifilm Wako Pure Chemical Corporation) at concentrations 1000 times higher than those of the exposure test; the compound solutions were added to the medium in the tissue bath and diluted appropriately.

Techniques:

Journal: Physiological Reports

Article Title: Induction of cardiac alternans in human iPS ‐derived cardiomyocytes through β‐adrenergic receptor stimulation

doi: 10.14814/phy2.70152

Figure Lengend Snippet: Effects of compounds on contractility of cardiac cell sheet tissues with C‐ALT cultured under atmospheric condition. (a–e) The typical contraction waveform, S/L ratio (each sample data), and CA (contraction amplitude) value (each sample data) of cardiac cell sheet tissue, cultured under atmospheric condition using Medium 199 Hanks' salt medium, before and after 30 min exposure to 1000 nM propranolol (S/L ratio [pre]; mean ± S.D. = 0.80 ± 0.12, S/L ratio [propranolol]; 0.99 ± 0.01, CA [pre]; 1.38 ± 0.23, CA [propranolol]; 1.05 ± 0.13, n = 8), 300 nM verapamil (S/L ratio [pre]; mean ± S.D. = 0.71 ± 0.16, S/L ratio [verapamil]; 0.94 ± 0.02, CA [pre]; 1.41 ± 0.24, CA [verapamil]; 0.49 ± 0.24, n = 6), 1000 nM thapsigargin (S/L ratio [pre]; mean ± S.D. = 0.78 ± 0.15, S/L ratio [thapsigargin]; 0.99 ± 0.01, CA [pre]; 1.25 ± 0.18, CA [thapsigargin]; 0.97 ± 0.19, n = 6), 30 nM ryanodine (S/L ratio [pre]; mean ± S.D. = 0.64 ± 0.11, S/L ratio [ryanodine]; 1.01 ± 0.02, CA [pre]; 1.30 ± 0.20, CA [ryanodine]; 0.55 ± 0.33, n = 6), and 300 nM blebbistatin (S/L ratio [pre]; mean ± S.D. = 0.65 ± 0.20, S/L ratio [blebbistatin]; 0.59 ± 0.20, CA [pre]; 1.42 ± 0.23, CA [blebbistatin]; 1.04 ± 0.16, n = 7), respectively. “Baseline” indicates no exposure to any compounds. “Pre” indicates that C‐ALT is induced by exposure to 1000 nM isoproterenol. CA was normalized to baseline.

Article Snippet: Isoproterenol (I0260; Tokyo Chemical Industry, Tokyo, Japan), propranolol (163‐24501; Fujifilm Wako Pure Chemical Corporation), verapamil (222‐00781; Fujifilm Wako Pure Chemical Corporation), ryanodine (ab120083; Abcam, Cambridge, UK), thapsigargin (10522; Cayman Chemical, MI, USA), omecamtiv mecarbil (CS‐0460; Chemscene LCC, NJ, USA), ivabradine (098–06921; Fujifilm Wako Pure Chemical Corporation), and blebbistatin (ax494682; Axol Bioscience) were dissolved in DMSO (031‐24051; Fujifilm Wako Pure Chemical Corporation) at concentrations 1000 times higher than those of the exposure test; the compound solutions were added to the medium in the tissue bath and diluted appropriately.

Techniques: Cell Culture

Journal: Physiological Reports

Article Title: Induction of cardiac alternans in human iPS ‐derived cardiomyocytes through β‐adrenergic receptor stimulation

doi: 10.14814/phy2.70152

Figure Lengend Snippet: Effects of compounds on contractility of cardiac cell sheet tissues under spontaneous beating. (a–c) The typical contraction waveform, S/L ratio (each sample data), CA (contraction amplitude) value (each sample data), and beat rate (beats/min, each sample data) of cardiac cell sheet tissue, cultured under atmospheric condition using Medium 199 Hanks' salt medium, before (baseline) and after 30 min exposure to 1000 nM isoproterenol (S/L ratio [baseline]; mean ± S.D. = 1.00 ± 0.00, S/L ratio [isoproterenol]; 0.71 ± 0.19, CA [isoproterenol]; 0.97 ± 0.09, Beat Rate [baseline]; 20.2 ± 4.5, Beat Rate [isoproterenol]; 67.9 ± 4.2, n = 10), 1000 nM propranolol (S/L ratio [pre]; mean ± S.D. = 0.61 ± 0.13, S/L ratio [propranolol]; 0.98 ± 0.02, CA [pre]; 1.01 ± 0.10, CA [propranolol]; 0.86 ± 0.04, Beat Rate [pre]; 65.6 ± 5.2, Beat Rate [propranolol]; 29.5 ± 4.2, n = 5), and 300 nM ivabradine (S/L ratio [pre]; mean ± S.D. = 0.62 ± 0.20, S/L ratio [ivabradine]; 0.87 ± 0.08, CA [pre]; 0.99 ± 0.09, CA [ivabradine]; 1.09 ± 0.06, Beat Rate [pre]; 66.7 ± 3.7, Beat Rate [ivabradine]; 12.1 ± 2.4, n = 5), respectively. Propranolol and ivabradine were exposed to C‐ALT‐induced cardiac cell sheet tissue by exposure to isoproterenol. “Baseline” indicates no exposure to any compounds. “Pre” indicates that C‐ALT is induced by one micromolar of isoproterenol exposure. CA was normalized to baseline.

Article Snippet: Isoproterenol (I0260; Tokyo Chemical Industry, Tokyo, Japan), propranolol (163‐24501; Fujifilm Wako Pure Chemical Corporation), verapamil (222‐00781; Fujifilm Wako Pure Chemical Corporation), ryanodine (ab120083; Abcam, Cambridge, UK), thapsigargin (10522; Cayman Chemical, MI, USA), omecamtiv mecarbil (CS‐0460; Chemscene LCC, NJ, USA), ivabradine (098–06921; Fujifilm Wako Pure Chemical Corporation), and blebbistatin (ax494682; Axol Bioscience) were dissolved in DMSO (031‐24051; Fujifilm Wako Pure Chemical Corporation) at concentrations 1000 times higher than those of the exposure test; the compound solutions were added to the medium in the tissue bath and diluted appropriately.

Techniques: Cell Culture